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Comparative genomics of the Arcobacter strains A. Finally, the new strain had an incomplete denitrification pathway where the end product was nitrite, which is different from other Arcobacter strains where the end product is ammonia.
Altogether, our study shows that traditional characterization in combination with a modern genomics approach can expand our knowledge on free-living Arcobacter, and that this complementary approach could also provide invaluable knowledge about the physiology and metabolic pathways in other Epsilonproteobacteria from various environments.
Keywords: Arcobacter, Epsilonproteobacteria, genomics, metabolism, hox hydrogenase, ferric citrate reduction Introduction Free-living, environmental Epsilonproteobacteria are found to be ubiquitous in marine and terrestrial habitats, such as hydrothermal systems, marine sediments, pelagic seawater, acid mine drainage, lakes, springs, sulfidic caves and hydrocarbon-rich ground water Campbell et al.
They are in general associated with sulfide rich environments where they play a key role in the cycling of carbon, nitrogen, and sulfur Campbell et al. Epsilonproteobacteria may also facilitate the colonization of other microbial groups due to detoxification of sulfur species, such as hydrogen sulfide Campbell et al.
Modern meta-omics technologies have revealed a wide distribution of free-living Epsilonproteobacteria Huber et al. Isolates are obtained within the genera Sulfurovum Inagaki et al. Many of the free-living Epsilonproteobacteria associated with hydrothermal vents can perform oxidation of hydrogen or sulfur species e. Of these, Arcobacter is the only genus with both pathogenic and free-living, non-pathogenic taxa. The first cultivated representatives of the genus Arcobacter were isolated from aborted bovine fetuses nearly 40 years ago Ellis et al.
As this genus includes many pathogens, the main focus of the characterization of first Arcobacter isolates was to distinguish between different species and survey the strains for resistance to antibiotics Neill et al. The genus Arcobacter was resolved by Vandamme et al. The new species have been isolated from a remarkably broad range of habitats, such as humans and animals Houf et al.
The metabolic potential of most environmental free-living Arcobacter species has not been examined in detail.
Libel is a statement or representation made in a permanent form such as picture, statues, sign, writing, caricatures, etc.
Section 3 of the Act provides that the broadcasting of words by means of radio communication shall be treated as publication in a permanent and therefore constitute libel. In Yousoupoff v MGM, the court held that the plaintiff was entitled to damages because the defamatory statement contained in the film made by the defendant is lible.
Slander is defamation in temporary form.
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Publication is usually made through spoken words or gestures. It is not actionable per se which the plaintiff has to prove actual or special damage such as financial loss. However, there are certain exceptions to this general rules. In order to prove defamation, the plaintiff must establish the elements of tort which are the words are defamatory, the words refer to the plaintiff and that the words have been published.
Firstly, the words are defamatory. Defamatory word must be distinguish from mere abusive words where in the case of Penfold v Wescote. In this case, the defendant called out why don't you come out you black guard, rascal, scoundrel, pen-fold, you are a thief.
The court held that only the word thief is defamatory because it is an imputation of a crime meanwhile black guard, rascal and scoundrel were mere abusive words. Words may be defamatory in two different ways.
Firstly, in natural and ordinary meaning. It means that the defamatory words can be understood by the ordinary intelligence people just using their general knowledge and common sense to make them look down, shun or avoid the plaintiff.
It was held that it is defamatory as it imputes that person as a big liar, untrustworthy and irresponsible. Secondly, innuendo where the statement becomes defamatory through inferences, special facts or circumstances known by the reader as in Tolley v JS Fry.
The second element is the words must refer to plaintiff. The test for this is if reasonable people would think that the language used by the defendant to be defamatory to the plaintiff, then the defendant is liable even if the defendant did not intend to refer to the plaintiff.
There was in fact a real Artemis Jones, the plaintiff, a lawyer in that town but his friends thought the story concerned the plaintiff. The court held that defamation was established as those who knew him understood the words as referring to him, although that was not intended to be so by the author and publisher.
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Thirdly, the words must be published. Publication means the words is communicated to a third party other than the defamed.
Words according to Section 2 of the Defamation Act include pictures, visual images, gestures, and other methods signifying meaning. Other relevant factor to establish publication are the language used by the defendant are understandable, the plaintiff can specify what the words about and the words were referring to the plaintiff.
This can be seen in the case of Normala Shamsudin v Keluarga Communication Sdn Bhd, where the defendant published an article concerning the plaintiff with the headline Mala tebus talak 3 Juta.
The court held that the word was defamatory because it indeed expose the plaintiff to hatred, ridicule, disesteem and more.
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Every repetition of a defamatory matter is a fresh publication and creates a fresh cause of action. If a defamatory article is published in the newspaper, the writer, editor, publisher and owner of the publishing company are all liable as in the case of Vincent Tan.
In applying to the present case, as the defamatory statement is in permanent form, thus it is libel.In the case S Pakianathan v Dr Jenni Ibrahim, the defendant alleged that the plaintiff had committed a criminal breach of trust amounting to RM Lastly, the defence of fair comment can be used by Mr Nose Best.
Promulgada de Hecho: Enero 13 de The court held that defamation was established as those who knew him understood the words as referring to him, although that was not intended to be so by the author and publisher. Hamilton Living Trust, its members, managers, employees, agents, servants, property owners, and volunteers in the operation, supervision, design or maintenance of the facility and the property it is on an Assumption of Risk.
Publication means the words is communicated to a third party other than the defamed.
It means that the defamatory words can be understood by the ordinary intelligence people just using their general knowledge and common sense to make them look down, shun or avoid the plaintiff. Me atridownloade en tal Slander is defamation in temporary form.
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