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Vocational Course in Industrial Microbiology is conducted as a part of the.. A historical overview of industrial fermentation process — traditional and modern A. Industrial Chemistry 6. Unit 1 Introduction to industrial microbiology and fermentation processes No.

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Shiva namaskar mantra pdf. Is there. The descendant of a single isolation in pure culture constitutes a strain. A strain is usually made up of succession of cultures and is often derived from a single colony. If a strain is derived from a single parental cell, it is termed a clone. Pour-plate technique Spread-plate technique Figure 3.

In it, the sample broth is streaked onto a dry agar surface in a series of non-overlapping streaks see figure 3. The process thins out the cells and at some point the cells are separated sufficiently apart to give rise to discrete colonies.

The method has some limitations in that psychrophiles or organisms that cannot withstand a temporary shock of C cannot survive. Besides, the isolated colonies remain embedded in the medium and subculturing them entails digging through the agar.

The single most important advantage in it is, it can be used for quantitative enumeration purpose also. A variation of this method is to transfer 0.

The plate is rotated gently in the shape of 8 to affect uniform mixing. Turntables can be used for the spreading. In manual method, a loopful of culture is transferred to the agar surface and spread uniformly with a bent glass rod called dally rod. To affect finer isolation, the residue in the rod is used to spread-plate yet another fresh plate see figure 3.


In this way, plates can be used for the spreading. As the spreading progresses, at some point the cells will be sufficiently apart to the affect isolation.

A small segment of mycelia that radiate outward can be aseptically cut out and place on a fresh medium for growth. With this method, an unequivocal selection of a single cell is possible. The method uses an instrument called micromanipulator a high resolution microscope fitted with manipulating ancillary and needs considerable expertise. During manipulation, the cell is first identified. Isolation takes place on an agar surface.

A sharp needle provided with the manipulator is brought close to the cell. The needle is allowed to rest on the agar surface near the cell so that a small dent is formed. Next, the field is shifted away from the needle tip.

The dented impression made by the needle during the shifting causes the cell to follow the course of the needle and thus gets separated. The cell is later cut out from the agar surface and subcultured see figure 3.

Figure 3.

Industrial Microbiology (Free Version)

As a result of this modification, progressive improvement in the yield can be anticipated. The most dramatic example is that of penicillin produced by Penicillium chrysogenum. The basic objective in improving strain is to make it reliable and efficient so that the microbial process becomes economical. After an organism is screened, it becomes necessary to increase the product yield from fermentation to minimize production costs. Product yield can be improved by: Developing a suitable medium for fermentation Refining the fermentation process Improving productivity of the strain Generally, major improvements arise from the last approach; all fermentation enterprises place considerable emphasis on this aspect.

The techniques and approaches used to genetically modify strains to increase the production of the desired product, collectively, is called strain improvement or strain development. It is important to note here that productivity is not alone the function of large yield. Productivity is also the function of microbial properties such as resistance to infection, temperature tolerance, resistance to analogs, genetic stability, appropriate flocculation characteristics, etc.

Strain improvement programs therefore place due emphasis on these aspects also. Mutation can occur either spontaneously or by deliberate induction.

The former method cannot be relied on, as it is terribly inefficient. A more direct approach is to use mutagenic agents mutagens. The frequency of mutation can be achieved at very high levels with this method. Of the remaining that are isolated by screening subculturing , only a small percentage will have actually mutated.

Not all the mutants will have the property as desired, though. Those that bear the desired property are identified, selected, and tested further using other special procedures.

It is carried out by suspending the cells in an acidic buffer and adding sodium nitrite to it. For example, oxidative deamination of cytosine results in uracil see figure 4.

This change causes replication errors. Mutation is carried out by suspending the cells in neutral buffer followed by addition of mutagen. The reaction may be stopped by using sodium thiosulfate or by dilution during plating.

Alkylating agents add alkyl groups to the nitrogen in the 7th position of the purine. This alkylation creates labile N-glycosidic bond that hydrolyzes to leave depurinated site see figure 4.

If not repaired immediately, any base may join to fill up the gap. This event leads to unmatched bases. It can produce very high mutant yields. It is also an alkylating agent and acts primarily at the replication fork. Our very first show was on 23rd February ! T he management, DJ's, engineers and backroom staff thank you for your continuous support.

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